Sec Form 3 Filing - Berriman John E @ Autolus Therapeutics plc - 2026-03-18

FORM 3 Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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1. Name and Address of Reporting Person Berriman John E 2. Issuer Name and Ticker or Trading Symbol Autolus Therapeutics plc [ AUTL] 5. Relationship of Reporting Person(s) to Issuer (Check all applicable) X __ Director _____ 10% Owner _____ Officer (give title below) _____ Other (specify below) (Last) (First) (Middle) C/O AUTOLUS THERAPEUTICS PLC, THE MEDIAWORKS, 191 WOOD LN, WHITE CITY 3. Date of Earliest Transaction (MM/DD/YY) 03/18/2026 (Street) LONDONW12 7FP 4. If Amendment, Date Original Filed (MM/DD/YY) 6. Individual or Joint/Group Filing (Check Applicable Line) __ X __ Form filed by One Reporting Person _____ Form filed by More than One Reporting Person (City) (State) (Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned 1.Title of Security (Instr. 3) 2. Transaction Date (MM/DD/YY) 2A. Deemed Execution Date, if any (MM/DD/YY) 3. Transaction Code (Instr. 8) 4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5) 5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4) 6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4) Code V Amount (A) or (D) Price Ordinary Shares ( 1 ) 136,331 D

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned ( e.g. , puts, calls, warrants, options, convertible securities) 1. Title of Derivative Security (Instr. 3) 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (MM/DD/YY) 3A. Deemed Execution Date, if any (MM/DD/YY) 4. Transaction Code (Instr. 8) 5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4, and 5) 6. Date Exercisable and Expiration Date (MM/DD/YY) 7. Title and Amount of Underlying Securities (Instr. 3 and 4) 8. Price of Derivative Security (Instr. 5) 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4) 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4) 11. Nature of Indirect Beneficial Ownership (Instr. 4) Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares Share Option (right to buy) $ 8.38 ( 2 ) 02/23/2028 American Depositary Shares 15,698 D Share Option (right to buy) $ 30.24 ( 2 ) 03/28/2029 American Depositary Shares 25,000 D Share Option (right to buy) $ 13.25 ( 2 ) 06/18/2030 American Depositary Shares 12,500 D Share Option (right to buy) $ 7.57 ( 2 ) 06/18/2031 American Depositary Shares 12,500 D Share Option (right to buy) $ 2.84 ( 2 ) 06/28/2032 American Depositary Shares 20,000 D Share Option (right to buy) $ 2.38 ( 2 ) 06/30/2033 American Depositary Shares 105,000 D Share Option (right to buy) $ 3.48 ( 2 ) 06/28/2034 American Depositary Shares 80,000 D Share Option (right to buy) $ 2.32 ( 3 ) 06/26/2035 American Depositary Shares 80,000 D

Reporting Owners Reporting Owner Name / Address Relationships Director 10% Owner Officer Other Berriman John E C/O AUTOLUS THERAPEUTICS PLC THE MEDIAWORKS, 191 WOOD LN, WHITE CITY LONDONW12 7FP X Signatures /s/ John Berriman 03/18/2026 Signature of Reporting Person Date

Explanation of Responses:

( 1 )The Ordinary Shares may be represented by American Depositary Shares, each of which currently represents one ordinary share. ( 2 )Fully vested and exercisable. ( 3 )This option vested or vests in twelve equal monthly installments commencing on July 26, 2025.

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).

** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).

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