Sec Form 3 Filing - Valerio Domenico @ ProQR Therapeutics N.V. - 2026-03-18

FORM 3 Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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1. Name and Address of Reporting Person Valerio Domenico 2. Issuer Name and Ticker or Trading Symbol ProQR Therapeutics N.V. [ PRQR] 5. Relationship of Reporting Person(s) to Issuer (Check all applicable) X __ Director _____ 10% Owner _____ Officer (give title below) _____ Other (specify below) (Last) (First) (Middle) PROQR THERAPEUTICS N.V., ZERNIKEDREEF 9 3. Date of Earliest Transaction (MM/DD/YY) 03/18/2026 (Street) LEIDEN2333 CK 4. If Amendment, Date Original Filed (MM/DD/YY) 6. Individual or Joint/Group Filing (Check Applicable Line) __ X __ Form filed by One Reporting Person _____ Form filed by More than One Reporting Person (City) (State) (Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned 1.Title of Security (Instr. 3) 2. Transaction Date (MM/DD/YY) 2A. Deemed Execution Date, if any (MM/DD/YY) 3. Transaction Code (Instr. 8) 4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5) 5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4) 6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4) Code V Amount (A) or (D) Price Ordinary Shares 420,729 D Ordinary Shares 304,963 I See footnote ( 1 )

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned ( e.g. , puts, calls, warrants, options, convertible securities) 1. Title of Derivative Security (Instr. 3) 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (MM/DD/YY) 3A. Deemed Execution Date, if any (MM/DD/YY) 4. Transaction Code (Instr. 8) 5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4, and 5) 6. Date Exercisable and Expiration Date (MM/DD/YY) 7. Title and Amount of Underlying Securities (Instr. 3 and 4) 8. Price of Derivative Security (Instr. 5) 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4) 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4) 11. Nature of Indirect Beneficial Ownership (Instr. 4) Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares Share Option (Right to Buy) $ 4.89 ( 2 ) 06/20/2026 Ordinary Shares 5,815 D Share Option (Right to Buy) $ 4.9 ( 2 ) 12/31/2026 Ordinary Shares 32,164 D Share Option (Right to Buy) $ 3.3 ( 2 ) 12/31/2027 Ordinary Shares 27,500 D Share Option (Right to Buy) $ 15.78 ( 2 ) 12/31/2028 Ordinary Shares 14,918 D Share Option (Right to Buy) $ 9.91 ( 2 ) 12/31/2029 Ordinary Shares 24,615 D Share Option (Right to Buy) $ 4.2 ( 2 ) 12/31/2030 Ordinary Shares 23,239 D Share Option (Right to Buy) $ 8.01 ( 2 ) 02/29/2032 Ordinary Shares 23,931 D Share Option (Right to Buy) $ 3.41 ( 3 ) 12/31/2032 Ordinary Shares 22,608 D Share Option (Right to Buy) $ 1.98 ( 4 ) 12/31/2033 Ordinary Shares 23,489 D Share Option (Right to Buy) $ 2.65 ( 5 ) 12/31/2034 Ordinary Shares 22,128 D Share Option (Right to Buy) $ 2.02 ( 6 ) 12/31/2035 Ordinary Shares 25,192 D

Reporting Owners Reporting Owner Name / Address Relationships Director 10% Owner Officer Other Valerio Domenico PROQR THERAPEUTICS N.V. ZERNIKEDREEF 9 LEIDEN2333 CK X Signatures /s/ Pieter Erik de Ridders, Attorney-in-Fact 03/18/2026 Signature of Reporting Person Date

Explanation of Responses:

( 1 )Shares held by Valerio family foundation, Stichting Administratiekantoor Endavit, for which the Reporting Person is the managing director. The Reporting Person disclaims beneficial ownership of such shares for purposes of Section 16 of the Securities Exchange Act of 1934, as amended (the "Exchange Act"), except to the extent of his pecuniary interest therein, if any. This report shall not be deemed an admission that the Reporting Person is a beneficial owner of such shares for purposes of Section 16 of the Exchange Act, or for any other purpose. ( 2 )Fully vested. ( 3 )25% of the shares subject to such option vest and become exercisable on January 1, 2024, and the remaining 75% vest in 12 substantially equal quarterly installments thereafter, subject to the Reporting Person's continuous service to the Issuer on each such date. ( 4 )25% of the shares subject to such option vest and become exercisable on January 1, 2025, and the remaining 75% vest in 12 substantially equal quarterly installments thereafter, subject to the Reporting Person's continuous service to the Issuer on each such date. ( 5 )25% of the shares subject to such option vest and become exercisable on January 1, 2026, and the remaining 75% vest in 12 substantially equal quarterly installments thereafter, subject to the Reporting Person's continuous service to the Issuer on each such date. ( 6 )25% of the shares subject to such option vest and become exercisable on January 1, 2027, and the remaining 75% vest in 12 substantially equal quarterly installments thereafter, subject to the Reporting Person's continuous service to the Issuer on each such date.

Remarks: Exhibit 24 - Power of Attorney

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).

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