Sec Form 4 Filing - KAYNE RICHARD A @ Kayne Anderson MLP Investment CO - 2018-05-01

FORM 4 Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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1. Name and Address of Reporting Person KAYNE RICHARD A 2. Issuer Name and Ticker or Trading Symbol Kayne Anderson MLP Investment CO [ KYN] 5. Relationship of Reporting Person(s) to Issuer (Check all applicable) _____ Director _____ 10% Owner _____ Officer (give title below) X __ Other (specify below) Affiliate of Investment Adviso (Last) (First) (Middle) 1800 AVENUE OF THE STARS, THIRD FLOOR 3. Date of Earliest Transaction (MM/DD/YY) 05/01/2018 (Street) LOS ANGELES, CA90067 4. If Amendment, Date Original Filed (MM/DD/YY) 6. Individual or Joint/Group Filing (Check Applicable Line) __ X __ Form filed by One Reporting Person _____ Form filed by More than One Reporting Person (City) (State) (Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned 1.Title of Security (Instr. 3) 2. Transaction Date (MM/DD/YY) 2A. Deemed Execution Date, if any (MM/DD/YY) 3. Transaction Code (Instr. 8) 4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5) 5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4) 6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4) Code V Amount (A) or (D) Price Common Stock 05/01/2018 P( 1 ) 14,265 D $ 17.5263 297,164 ( 2 ) I See footnotes Common Stock 176,725 D

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned ( e.g. , puts, calls, warrants, options, convertible securities) 1. Title of Derivative Security (Instr. 3) 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (MM/DD/YY) 3A. Deemed Execution Date, if any (MM/DD/YY) 4. Transaction Code (Instr. 8) 5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4, and 5) 6. Date Exercisable and Expiration Date (MM/DD/YY) 7. Title and Amount of Underlying Securities (Instr. 3 and 4) 8. Price of Derivative Security (Instr. 5) 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4) 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4) 11. Nature of Indirect Beneficial Ownership (Instr. 4) Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares

Reporting Owners Reporting Owner Name / Address Relationships Director 10% Owner Officer Other KAYNE RICHARD A 1800 AVENUE OF THE STARS, THIRD FLOOR LOS ANGELES, CA90067 Affiliate of Investment Adviso Signatures Richard A. Kayne 05/08/2018 Signature of Reporting Person Date

Explanation of Responses:

( 1 )Negotiated transaction between KA Associates Inc., an affiliate of the investment advisor of the Issuer (see footnote 2), and Kevin S. McCarthy, Chairman of the Issuer. Price determined based on the 10-day VWAP for the period April 16-30, 2018. ( 2 )A total of 285,929 shares are owned by Kayne Anderson Capital Advisors, L.P. ("KACALP"), Richard Kayne is the majority owner and chairman of KACALP. A total of 11,235 shares are owned by KA Associates, Inc. ("KAA"), a FINRA registered broker-dealer. Richard Kayne is the majority owner of KAA. Mr. Kayne disclaims beneficial ownership of all shares held or controlled by KACALP and KAA except to the extent of his pecuniary interest therein.

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).

** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).

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