Sec Form 3 Filing - Raffin Thomas A. @ Akoya Biosciences, Inc. - 2021-04-20

FORM 3 Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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1. Name and Address of Reporting Person Raffin Thomas A. 2. Issuer Name and Ticker or Trading Symbol Akoya Biosciences, Inc. [ AKYA] 5. Relationship of Reporting Person(s) to Issuer (Check all applicable) X __ Director X __ 10% Owner _____ Officer (give title below) _____ Other (specify below) (Last) (First) (Middle) 100 CAMPUS DRIVE, 6TH FLOOR, 3. Date of Earliest Transaction (MM/DD/YY) 04/20/2021 (Street) MARLBOROUGH, MA01752 4. If Amendment, Date Original Filed (MM/DD/YY) 6. Individual or Joint/Group Filing (Check Applicable Line) _____ Form filed by One Reporting Person __ X __ Form filed by More than One Reporting Person (City) (State) (Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned 1.Title of Security (Instr. 3) 2. Transaction Date (MM/DD/YY) 2A. Deemed Execution Date, if any (MM/DD/YY) 3. Transaction Code (Instr. 8) 4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5) 5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4) 6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4) Code V Amount (A) or (D) Price Common Stock 429,184 D Common Stock 14,134,162 I See Footnote ( 1 ) Common Stock 1,541,085 I See Footnote ( 2 )

Expiration Date Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned ( e.g. , puts, calls, warrants, options, convertible securities) 1. Title of Derivative Security (Instr. 3) 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (MM/DD/YY) 3A. Deemed Execution Date, if any (MM/DD/YY) 4. Transaction Code (Instr. 8) 5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4, and 5) 6. Date Exercisable and Expiration Date (MM/DD/YY) 7. Title and Amount of Underlying Securities (Instr. 3 and 4) 8. Price of Derivative Security (Instr. 5) 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4) 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4) 11. Nature of Indirect Beneficial Ownership (Instr. 4) Code V (A) (D) Date Exercisable Title Amount or Number of Shares

Reporting Owners Reporting Owner Name / Address Relationships Director 10% Owner Officer Other Raffin Thomas A. 100 CAMPUS DRIVE, 6TH FLOOR MARLBOROUGH, MA01752 X X MACKOWSKI J MATTHEW 360 POST STREET, SUITE 601 SAN FRANCISCO, CA94108 X Lashkari Deval 360 POST STREET, SUITE 601 SAN FRANCISCO, CA94108 X Telegraph Hill Partners III, L.P. 360 POST STREET, SUITE 601 SAN FRANCISCO, CA94108 X THP III Affiliates Fund, LLC 360 POST STREET, SUITE 601 SAN FRANCISCO, CA94108 X Telegraph Hill Partners III Investment Management, LLC 360 POST STREET SUITE 601 SAN FRANCISCO, CA94108 X Telegraph Hill Partners Management Company, LLC 360 POST STREET SUITE 601 SAN FRANCISCO, CA94108 X Signatures /s/ Thomas A. Raffin, Brian McKelligon, Attorney-in-Fact 04/20/2021 Signature of Reporting Person Date

Explanation of Responses:

( 1 )Shares held directly by Telegraph Hill Partners III, L.P. ("THP III"). Telegraph Hill Partners III Investment Management, LLC ("THP IM") is the general partner of THP III. Telegraph Hill Partners Management Company, LLC ("THPMC") is the manager of THP IM. J. Matthew Mackowski, Dr. Thomas A. Raffin and Deval Lashkari are each managers of THPMC and are deemed to have beneficial ownership of the shares held by THP III. ( 2 )Shares held directly by THP III Affiliates Fund, LLC ("THP III AFF"). THP IM is the manager of THP III AFF. THPMC is the manager of THP IM. J. Matthew Mackowski, Dr. Thomas A. Raffin and Deval Lashkari are each managers of THPMC and are deemed to have beneficial ownership of the shares held by THP III Affiliates.

Remarks: Exhibit 24.1 Power of Attorney. Thomas A. Raffin is a member of the board of directors of Akoya Biosciences, Inc.

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).

** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).

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